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The significance of the additional FBXO22-NSD2 interaction surface, which is not interacting with the compound, was further assessed in a proximity-based Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) assay which uses a biotinylated version of the FBXO22 FIST domain and His-tagged NSD2 PWWP1

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These vacuoles, similar to those in posterior capsular opacification, represent enlarged migratory epithelial cells, also known as bladder or Wedl cells

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